Molecular Identification of Secreted Effector Genes Involved in African Fusarium oxysporum f.sp. elaeidis Strains Pathogenesis During Screening Nigerian Susceptible and Tolerant Oil Palm (Elaeis guineensis Jacq.) Genotypes.
Identifieur interne : 000106 ( Main/Exploration ); précédent : 000105; suivant : 000107Molecular Identification of Secreted Effector Genes Involved in African Fusarium oxysporum f.sp. elaeidis Strains Pathogenesis During Screening Nigerian Susceptible and Tolerant Oil Palm (Elaeis guineensis Jacq.) Genotypes.
Auteurs : Nnamdi Ifechukwude Chidi [Nigeria] ; Adedotun Adeyinka Adekunle [Nigeria] ; Temitope Oluwaseun Samuel [Nigeria] ; Emmanuel Ifechukwude Eziashi [Nigeria]Source :
- Frontiers in cellular and infection microbiology [ 2235-2988 ] ; 2020.
Abstract
Fusarium wilt is caused by Fusarium oxysporum f. sp. elaeidis, and constitutes a severe threat to the oil palm industry in Africa. This study is aimed at surveying, identifying the secreted effector genes responsible for virulence during pathogenesis, and investigating the level of genetic diversity and cluster resolutions of alleles accountable for virulence in pathogenic strains of F. oxysporum f.sp. elaeidis from African countries. Fifty-eight fungal strains were isolated from acute and chronic Fusarium wilt diseased oil palms in Nigeria, Ghana and Cameroon. Morphological and sequencing analysis of the Internal Transcribed Spacer (ITS) region grouped all strains into nine dominant strains with a majority (41.37%) belonging to F. oxysporum, followed by F. solani (20.68%), F. equiseti (20.68%), F. verticilliodes (5.17%), F. proliferatum (3.44%), F. chlamydosporum (3.44%), F. nelsonii (1.72%), Fomes fomentarius, and Penicillium simplicissimum (1.72%). Disease incidence and severity showed varying levels of virulence with some Fusarium strains causing severe symptoms while others exhibited slight symptoms. ISSR evaluation disclosed a considerable level of genetic diversity among pathogenic F. oxysporum f.sp. elaeidis strains. Molecular characterization using defense gene primers revealed that the oil palm genotypes screened did not amplify defense genes. During pathogenesis, Fusarium strains produced GMC oxidoreductases, hypothetical proteins, FOIG 16629, FOXG 14258, and Pyranose dehydrogenase 3-like proteins using virulent effector gene primers. Polymerase Chain Reaction analysis using specific gene primers revealed that PRK02106, beta and BetA effector genes were secreted explicitly by F. oxysporum f.sp. elaeidis (4) and F. oxysporum f.sp. elaeidis (CRT) strains while screening tolerant oil palm genotypes. During screening susceptible oil palm genotypes, F. oxysporum f.sp. elaeidis (4) and F. oxysporum f.sp. elaeidis (CRT) strains produced FGGY_L-XK1, PRK10939, FGGY_N1, XylB1, XylB2, FGGY_L-XK2, XylB3, FGGY_N2, and XylB4 effector genes. Identifying these effector genes will provide the platform to study the basis of pathogenesis which will help breeders to modify breeding techniques for the improvement of oil palm genotypes in order to reduce oil palm loss in plantations and enhance food security.
DOI: 10.3389/fcimb.2020.552394
PubMed: 33123493
PubMed Central: PMC7573130
Affiliations:
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Genotypes.</title><author><name sortKey="Chidi, Nnamdi Ifechukwude" sort="Chidi, Nnamdi Ifechukwude" uniqKey="Chidi N" first="Nnamdi Ifechukwude" last="Chidi">Nnamdi Ifechukwude Chidi</name><affiliation wicri:level="1"><nlm:affiliation>Plant Pathology Division, Nigerian Institute for Oil Palm Research (NIFOR), Benin, Nigeria.</nlm:affiliation><country xml:lang="fr">Nigeria</country><wicri:regionArea>Plant Pathology Division, Nigerian Institute for Oil Palm Research (NIFOR), Benin</wicri:regionArea><wicri:noRegion>Benin</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Botany, University of Lagos, Akoka, Nigeria.</nlm:affiliation><country xml:lang="fr">Nigeria</country><wicri:regionArea>Department of Botany, University of Lagos, Akoka</wicri:regionArea><wicri:noRegion>Akoka</wicri:noRegion></affiliation></author><author><name sortKey="Adekunle, Adedotun Adeyinka" sort="Adekunle, Adedotun Adeyinka" uniqKey="Adekunle A" first="Adedotun Adeyinka" last="Adekunle">Adedotun Adeyinka Adekunle</name><affiliation wicri:level="1"><nlm:affiliation>Plant Pathology Division, Nigerian Institute for Oil Palm Research (NIFOR), Benin, Nigeria.</nlm:affiliation><country xml:lang="fr">Nigeria</country><wicri:regionArea>Plant Pathology Division, Nigerian Institute for Oil Palm Research (NIFOR), Benin</wicri:regionArea><wicri:noRegion>Benin</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Botany, University of Lagos, Akoka, Nigeria.</nlm:affiliation><country xml:lang="fr">Nigeria</country><wicri:regionArea>Department of Botany, University of Lagos, Akoka</wicri:regionArea><wicri:noRegion>Akoka</wicri:noRegion></affiliation></author><author><name sortKey="Samuel, Temitope Oluwaseun" sort="Samuel, Temitope Oluwaseun" uniqKey="Samuel T" first="Temitope Oluwaseun" last="Samuel">Temitope Oluwaseun Samuel</name><affiliation wicri:level="1"><nlm:affiliation>Plant Pathology Division, Nigerian Institute for Oil Palm Research (NIFOR), Benin, Nigeria.</nlm:affiliation><country xml:lang="fr">Nigeria</country><wicri:regionArea>Plant Pathology Division, Nigerian Institute for Oil Palm Research (NIFOR), Benin</wicri:regionArea><wicri:noRegion>Benin</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Botany, University of Lagos, Akoka, Nigeria.</nlm:affiliation><country xml:lang="fr">Nigeria</country><wicri:regionArea>Department of Botany, University of Lagos, Akoka</wicri:regionArea><wicri:noRegion>Akoka</wicri:noRegion></affiliation></author><author><name sortKey="Eziashi, Emmanuel Ifechukwude" sort="Eziashi, Emmanuel Ifechukwude" uniqKey="Eziashi E" first="Emmanuel Ifechukwude" last="Eziashi">Emmanuel Ifechukwude Eziashi</name><affiliation wicri:level="1"><nlm:affiliation>Plant Pathology Division, Nigerian Institute for Oil Palm Research (NIFOR), Benin, Nigeria.</nlm:affiliation><country xml:lang="fr">Nigeria</country><wicri:regionArea>Plant Pathology Division, Nigerian Institute for Oil Palm Research (NIFOR), Benin</wicri:regionArea><wicri:noRegion>Benin</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Botany, University of Lagos, Akoka, Nigeria.</nlm:affiliation><country xml:lang="fr">Nigeria</country><wicri:regionArea>Department of Botany, University of Lagos, Akoka</wicri:regionArea><wicri:noRegion>Akoka</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Plant Pathology Division, Shea Tree Crop Department, Nigerian Institute for Oil Palm Research (NIFOR), Benin, Nigeria.</nlm:affiliation><country xml:lang="fr">Nigeria</country><wicri:regionArea>Plant Pathology Division, Shea Tree Crop Department, Nigerian Institute for Oil Palm Research (NIFOR), Benin</wicri:regionArea><wicri:noRegion>Benin</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in cellular and infection microbiology</title><idno type="eISSN">2235-2988</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Fusarium wilt is caused by <i>Fusarium oxysporum</i> f. sp. <i>elaeidis</i>, and constitutes a severe threat to the oil palm industry in Africa. This study is aimed at surveying, identifying the secreted effector genes responsible for virulence during pathogenesis, and investigating the level of genetic diversity and cluster resolutions of alleles accountable for virulence in pathogenic strains of <i>F. oxysporum</i> f.sp. <i>elaeidis</i> from African countries. Fifty-eight fungal strains were isolated from acute and chronic Fusarium wilt diseased oil palms in Nigeria, Ghana and Cameroon. Morphological and sequencing analysis of the Internal Transcribed Spacer (ITS) region grouped all strains into nine dominant strains with a majority (41.37%) belonging to <i>F. oxysporum</i>, followed by <i>F. solani</i> (20.68%), <i>F. equiseti</i> (20.68%), <i>F. verticilliodes</i> (5.17%), <i>F. proliferatum</i> (3.44%), <i>F. chlamydosporum</i> (3.44%), <i>F. nelsonii</i> (1.72%), <i>Fomes fomentarius</i>, and <i>Penicillium simplicissimum</i> (1.72%). Disease incidence and severity showed varying levels of virulence with some Fusarium strains causing severe symptoms while others exhibited slight symptoms. ISSR evaluation disclosed a considerable level of genetic diversity among pathogenic <i>F. oxysporum</i> f.sp. <i>elaeidis</i> strains. Molecular characterization using defense gene primers revealed that the oil palm genotypes screened did not amplify defense genes. During pathogenesis, <i>Fusarium</i> strains produced GMC oxidoreductases, hypothetical proteins, <i>FOIG 16629, FOXG 14258</i>, and Pyranose dehydrogenase 3-like proteins using virulent effector gene primers. Polymerase Chain Reaction analysis using specific gene primers revealed that <i>PRK02106, beta</i> and <i>BetA</i> effector genes were secreted explicitly by <i>F. oxysporum</i> f.sp. <i>elaeidis</i> (4) and <i>F. oxysporum</i> f.sp. <i>elaeidis</i> (CRT) strains while screening tolerant oil palm genotypes. During screening susceptible oil palm genotypes, <i>F. oxysporum</i> f.sp. <i>elaeidis</i> (4) and <i>F. oxysporum</i> f.sp. <i>elaeidis</i> (CRT) strains produced <i>FGGY_L-XK1, PRK10939, FGGY_N1, XylB1, XylB2, FGGY_L-XK2, XylB3, FGGY_N2</i>, and <i>XylB4</i> effector genes. Identifying these effector genes will provide the platform to study the basis of pathogenesis which will help breeders to modify breeding techniques for the improvement of oil palm genotypes in order to reduce oil palm loss in plantations and enhance food security.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">33123493</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>31</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">2235-2988</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Frontiers in cellular and infection microbiology</Title><ISOAbbreviation>Front Cell Infect Microbiol</ISOAbbreviation></Journal><ArticleTitle>Molecular Identification of Secreted Effector Genes Involved in African <i>Fusarium oxysporum</i> f.sp. <i>elaeidis</i> Strains Pathogenesis During Screening Nigerian Susceptible and Tolerant Oil Palm (<i>Elaeis guineensis</i> Jacq.) Genotypes.</ArticleTitle><Pagination><MedlinePgn>552394</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fcimb.2020.552394</ELocationID><Abstract><AbstractText>Fusarium wilt is caused by <i>Fusarium oxysporum</i> f. sp. <i>elaeidis</i>, and constitutes a severe threat to the oil palm industry in Africa. This study is aimed at surveying, identifying the secreted effector genes responsible for virulence during pathogenesis, and investigating the level of genetic diversity and cluster resolutions of alleles accountable for virulence in pathogenic strains of <i>F. oxysporum</i> f.sp. <i>elaeidis</i> from African countries. Fifty-eight fungal strains were isolated from acute and chronic Fusarium wilt diseased oil palms in Nigeria, Ghana and Cameroon. Morphological and sequencing analysis of the Internal Transcribed Spacer (ITS) region grouped all strains into nine dominant strains with a majority (41.37%) belonging to <i>F. oxysporum</i>, followed by <i>F. solani</i> (20.68%), <i>F. equiseti</i> (20.68%), <i>F. verticilliodes</i> (5.17%), <i>F. proliferatum</i> (3.44%), <i>F. chlamydosporum</i> (3.44%), <i>F. nelsonii</i> (1.72%), <i>Fomes fomentarius</i>, and <i>Penicillium simplicissimum</i> (1.72%). Disease incidence and severity showed varying levels of virulence with some Fusarium strains causing severe symptoms while others exhibited slight symptoms. ISSR evaluation disclosed a considerable level of genetic diversity among pathogenic <i>F. oxysporum</i> f.sp. <i>elaeidis</i> strains. Molecular characterization using defense gene primers revealed that the oil palm genotypes screened did not amplify defense genes. During pathogenesis, <i>Fusarium</i> strains produced GMC oxidoreductases, hypothetical proteins, <i>FOIG 16629, FOXG 14258</i>, and Pyranose dehydrogenase 3-like proteins using virulent effector gene primers. Polymerase Chain Reaction analysis using specific gene primers revealed that <i>PRK02106, beta</i> and <i>BetA</i> effector genes were secreted explicitly by <i>F. oxysporum</i> f.sp. <i>elaeidis</i> (4) and <i>F. oxysporum</i> f.sp. <i>elaeidis</i> (CRT) strains while screening tolerant oil palm genotypes. During screening susceptible oil palm genotypes, <i>F. oxysporum</i> f.sp. <i>elaeidis</i> (4) and <i>F. oxysporum</i> f.sp. <i>elaeidis</i> (CRT) strains produced <i>FGGY_L-XK1, PRK10939, FGGY_N1, XylB1, XylB2, FGGY_L-XK2, XylB3, FGGY_N2</i>, and <i>XylB4</i> effector genes. Identifying these effector genes will provide the platform to study the basis of pathogenesis which will help breeders to modify breeding techniques for the improvement of oil palm genotypes in order to reduce oil palm loss in plantations and enhance food security.</AbstractText><CopyrightInformation>Copyright © 2020 Chidi, Adekunle, Samuel and Eziashi.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chidi</LastName><ForeName>Nnamdi Ifechukwude</ForeName><Initials>NI</Initials><AffiliationInfo><Affiliation>Plant Pathology Division, Nigerian Institute for Oil Palm Research (NIFOR), Benin, Nigeria.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Botany, University of Lagos, Akoka, Nigeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Adekunle</LastName><ForeName>Adedotun Adeyinka</ForeName><Initials>AA</Initials><AffiliationInfo><Affiliation>Plant Pathology Division, Nigerian Institute for Oil Palm Research (NIFOR), Benin, Nigeria.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Botany, University of Lagos, Akoka, Nigeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Samuel</LastName><ForeName>Temitope Oluwaseun</ForeName><Initials>TO</Initials><AffiliationInfo><Affiliation>Plant Pathology Division, Nigerian Institute for Oil Palm Research (NIFOR), Benin, Nigeria.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Botany, University of Lagos, Akoka, Nigeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Eziashi</LastName><ForeName>Emmanuel Ifechukwude</ForeName><Initials>EI</Initials><AffiliationInfo><Affiliation>Plant Pathology Division, Nigerian Institute for Oil Palm Research (NIFOR), Benin, Nigeria.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Botany, University of Lagos, Akoka, Nigeria.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Plant Pathology Division, Shea Tree Crop Department, Nigerian Institute for Oil Palm Research (NIFOR), Benin, Nigeria.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Cell Infect Microbiol</MedlineTA><NlmUniqueID>101585359</NlmUniqueID><ISSNLinking>2235-2988</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Fusarium oxysporum f.sp. elaeidis</Keyword><Keyword MajorTopicYN="N">Fusarium wilt</Keyword><Keyword MajorTopicYN="N">genetic diversity</Keyword><Keyword MajorTopicYN="N">incidence</Keyword><Keyword MajorTopicYN="N">oil palm</Keyword><Keyword MajorTopicYN="N">pathogenesis</Keyword><Keyword MajorTopicYN="N">severity</Keyword><Keyword MajorTopicYN="N">virulence</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>04</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>08</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>30</Day><Hour>5</Hour><Minute>55</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33123493</ArticleId><ArticleId IdType="doi">10.3389/fcimb.2020.552394</ArticleId><ArticleId 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last="Chidi">Nnamdi Ifechukwude Chidi</name><name sortKey="Eziashi, Emmanuel Ifechukwude" sort="Eziashi, Emmanuel Ifechukwude" uniqKey="Eziashi E" first="Emmanuel Ifechukwude" last="Eziashi">Emmanuel Ifechukwude Eziashi</name><name sortKey="Eziashi, Emmanuel Ifechukwude" sort="Eziashi, Emmanuel Ifechukwude" uniqKey="Eziashi E" first="Emmanuel Ifechukwude" last="Eziashi">Emmanuel Ifechukwude Eziashi</name><name sortKey="Eziashi, Emmanuel Ifechukwude" sort="Eziashi, Emmanuel Ifechukwude" uniqKey="Eziashi E" first="Emmanuel Ifechukwude" last="Eziashi">Emmanuel Ifechukwude Eziashi</name><name sortKey="Samuel, Temitope Oluwaseun" sort="Samuel, Temitope Oluwaseun" uniqKey="Samuel T" first="Temitope Oluwaseun" last="Samuel">Temitope Oluwaseun Samuel</name><name sortKey="Samuel, Temitope Oluwaseun" sort="Samuel, Temitope Oluwaseun" uniqKey="Samuel T" first="Temitope Oluwaseun" last="Samuel">Temitope Oluwaseun Samuel</name></country></tree></affiliations></record>Pour manipuler ce document sous 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